The present invention addresses the problem of producing a functional intestinal organoid from pluripotent stem cells. An intestinal organoid is produced from pluripotent stem cells through the following steps: (1) a step for differentiating the pluripotent stem cells into endoderm-like cells； (2) a step for differentiating the endoderm-like cells obtained in step (1) into intestinal stem cell-like cells； (3) a step for culturing the intestinal stem cell-like cells obtained in step (2) to form a spheroid； and (4) a step for differentiating the spheroid formed in step (3) to form an intestinal organoid, wherein culturing is carried out in the presence of, in addition to an epidermal growth factor, a BMP inhibitor and a Wnt signal activator, a MEK1/2 inhibitor, a DNA methylation inhibitor, a TGFß receptor inhibitor and a ?-secretase inhibitor.
