The present invention relates to compositions and methods for providing and using markers of aphid resistant germplasm, particularly for identifying aphid resistance germplasm in soybean plants. Specifically, the inventions related to providing aphid resistant germplasm identified by markers associated with decreased damage from aphid feeding, as well as enhanced tolerance to aphid infestation of soybean plants. More particularly, the invention relates to compositions and methods for using genetic markers in breeding methods for producing plants with increased resistance to aphid damage and tolerance, while retaining and acquiring desired agronomic traits. Additionally, markers were developed for fine mapping of aphid resistance genes including allele specific contributions to plants for breeding soybean plants with increased aphid resistance. Furthermore, the invention relates to plants produced by these breeding programs, including plants with having aphid resistant genes between the molecular markers identifying aphid resistant genes, for use in commercial soybean production.