Disclosed is a method of preparing a compound having a glycerolic backbone, at least one oxidized moiety-containing residue attached to the glycerolic backbone via an ether bond and a phosphorus-containing moiety attached to the glycerolic backbone, the compound having the general Formula II the method comprising: providing a first compound having a glycerolic backbone and a phosphorus containing moiety, said first compound having general Formula I: providing a fifth compound having at least one oxidized moiety and at least one fourth reactive group reacting said first compound and said fifth compound in the presence of a base selected from the group consisting of potassium hydroxide and sodium hydroxide, and removing water formed by reaction of said base by azeotropic distillation, to thereby obtain a reaction mixture containing a compound having a glycerolic backbone and at least one oxidized moiety-containing residue attached to the glycerolic backbone via an ether bond and isolating the compound having a glycerolic backbone and at least one oxidized moiety-containing residue attached to the glycerolic backbone via an ether bond, removing said protecting group and reacting said compound having said at least one oxidized moiety-containing residue attached to said glycerolic backbone with a phosphorus-containing moiety, to thereby obtain said compound having a glycerolic backbone, at least one oxidized moiety containing residue attached to the glycerolic backbone via an ether bond and a phosphorus containing moiety attached to the glycerolic backbone.
