Method for removing biofilms present on a substrate, characterized by comprising the following steps: a) preparation of a detergent component comprising a sequestering agent and a wetting agent and a dispersing agent, and an enzyme component containing at least one protease, at least one laccase and at least one polysaccharidase, b) dissolving the detergent component in an aqueous phase, c) dissolving the enzyme component in the solution formed in step b) to form a solution of a composition for removing biofilms present on a substrate, wherein said solution formed has a pH between 6.5 and 7.5, or b ') dissolving the enzyme component in an aqueous phase, c') dissolving the detergent component in the solution formed in step b '), to form a solution for removing biofilms, wherein said solution formed has a pH between 6.5 and 7.5, d) applies tion of the solution of said composition, formed in step c) or c ') on the substrate for a predetermined period of time, in particular between 15 minutes and 4 hours, e) adding a basic solution after said d application) of the solution of said composition on said substrate, during said predetermined period of time, in order to raise the pH to about 8 to 9.Método para la eliminación de biopelículas presentes sobre un sustrato, caracterizado por que comprende las siguientes etapas: a) preparación de un componente detergente que comprende un agente secuestrante, así como un agente humectante y un agente dispersante, y de un componente enzimático que contiene al menos una proteasa, al menos una lacasa y al menos una polisacaridasa, b) disolver el componente detergente en una fase acuosa, c) disolver el componente enzimático en la solución formada en la etapa b), para formar una solución de una composición para la eliminación de las biopelículas presentes sobre un sustrato, en donde dicha solución formada tiene un pH comprendido entre 6,5 y 7,5, o b') disolver el componente enzimático en una fase acuosa, c') disolver el componente deterg
