The present invention relates to an antibacterial material comprising a tyndallized dead lactic acid bacteria lyophilizate and a dried tyndallized fermented filtrate prepared by a process comprising the steps of tyndallizing a fermented broth of lactic acid bacteria and then separating the tyndallized fermented broth into a tyndallized fermented filtrate and a tyndallized dead lactic acid bacteria-containing solution； freeze-drying the tyndallized dead lactic acid bacteria-containing solution so as to form a tyndallized dead lactic acid bacteria lyophilizate； fluidized-bed-drying or freeze-drying the tyndallized fermented filtrate so as to from a dried tyndallized fermented filtrate； and mixing the tyndallized dead lactic acid bacteria lyophilizate and the dried tyndallized fermented filtrate, and a preparing method thereof. The antibacterial material comprising the tyndallized dead lactic acid bacteria lyophilizate, according to the present invention, may have a homogeneous appearance and an excellent antibacterial activity, and thus may be appropriately used to prepare various medicines, foods, feeds, and cosmetics which require antibacterial activity and intestinal regulation.
