The present invention relates to a functional fermentation material having an effect of anti-inflammatory and anti-helicobacter fermented broccoli with lactic acid bacteria, and a method for preparing the same, more specifically, Lactobacillus plantarum MG207; Lactobacillus paracasei MG310; Lactobacillus casei MG311; Lactobacillus acidophilus MG501; Streptococcus thermophilus MG510; And Bifidobacterium longum MG723 relates to a functional fermentation material having the effect of anti-inflammatory and anti-helicobacter fermented broccoli with lactic acid bacteria, characterized in that any one or more selected from the group consisting of. In addition, to minimize the destruction of sulforaphane and at the same time to effectively kill the general bacteria, adding 5 ~ 10wt% broccoli to distilled water; 25-35 minutes sterilization at 75-85; 2 hours to 3 hours at room temperature; It relates to a method for producing a functional fermentation material having an effect of anti-inflammatory and anti-Helicobacter fermented broccoli, characterized in that it comprises a step of sterilizing 25 to 35 minutes at 75 to 85 again. On the other hand, broccoli is fermented with lactic acid bacteria to suppress the proliferation of Helicobacter pylori bacteria as well as to increase the cytokine material that inhibits inflammation that can be caused by Helicobacter bacteria and to reduce the cytokine that activates inflammation. It is about. Furthermore, the present invention relates to a method of treating a basic cryoprotectant to increase the survival rate of bacteria of a functional fermentation substance having an effect of anti-inflammatory and anti-helicobacter fermented broccoli with lactic acid bacteria. Anti-inflammatory and anti-Helicobacter effective fermentation broccoli of the present invention with lactic acid bacteria through the functional fermentation and its manufacturing method to prevent various side effects caused by the abuse of antibiotics, and of course,