PURPOSE: A lipase inhibitor containing an Ecklonia cava ethyl acetate fraction or dieckol isolated from the fraction is provided to ensure thermal stability and pH stability and to be used in a pharmaceutical product and health foods for suppressing obesity.CONSTITUTION: A lipase inhibitor contains Ecklonia cava ethyl acetate fraction and dieckol as an active ingredient. A composition for suppressing obesity contains the fraction and dieckol as an active ingredient. A method for preparing the Ecklonia cava ethyl acetate fraction or dieckol isolated from the same comprises: a step of adding Ecklonia cava to a mixture solvent of water and C1-C4 alcohol and extracting at room temperature for 24 hours(three times) a step of centrifuging the extract and collecting supernatant a step of filtering and concentrating the supernatant by reduced pressure a step of suspending the Ecklonia cava ethanol extract in distilled water a step of adding n-hexane, chloroform, ethyl acetate, and butanol to the extract in order and a step of concentrating and drying the fraction.COPYRIGHT KIPO 2013[Reference numerals] (AA) Ecklonia cava ethanol extract (BB) n-hexane (CC) Hexane fraction (H) (DD,II,LL) Water layer (EE) Chloroform (FF) Silicagel column chromatography (GG) Methylene chloride:ethanol (HH) Chloroform fraction(CH) (JJ) Ethyl acetate (KK) Ethyl acetate fraction(EA) (MM) Butanol (NN,RR) Silicagel column chromatography (OO) Ethyl acetate:methanol (PP) Butanol fraction(Bu) (QQ) Water fraction(Wa) (SS) Chloroform:ethanol (TT) Reverse phase HPLC (UU) Solvent:36% ACN (VV) Flux: 1mL/min, Detector: 254nm본 발명은 감태 에틸아세테이트 분획물 또는 이로부터 분리된 디엑콜을 유효성분으로 함유하는 리파아제 저해제에 관한 것이다. 본 발명에 따른 감태 에틸아세테이트 분획물 또는 이로부터 분리된 디엑콜은 리파아제 저해활성이 우수하고, 열 안정성 및 pH 안정성이 우수하여, 고열 처리 및 pH 처리를 동반하는 식품 가공 공정에 효과적으로 이용할 수 있으며, 비만 억제에 유용한 의약품 및 건강기능식품으로도 사용될 수 있다.
