The invention discloses a dipolyiridoid glycoside compound and a preparation method and an application thereof. The preparation method comprises the following steps: (1) taking a Reduning injection product, carrying out column chromatography separation by a HP-20 macroporous resin, sequentially eluting by water, 30% ethanol, 90-100% ethanol in a gradient manner, and respectively collecting the eluants and concentrating to obtain a 90-100% ethanol eluant part and (2) sequentially carrying out silica gel column chromatography and ODS (Octadecyl Silane) column chromatography separation and half preparative liquid phase HPLC (High Performance Liquid Chromatography) separation on the 90-100% ethanol eluant part to obtain the compound disclosed by the invention. The compound disclosed by the invention can be used for treating hand-foot-and-mouth disease.
