The present invention relates to a method for obtaining a mixture of biological factors isolated from colostrum, characterized by one or more step of filtration and concentration that allow the separation of large proteins and pathogenic microorganisms from the permeate, and to maintain the biological factors of interest in the retentate. The present invention further comprises a method for obtaining a mixture of biological factors comprising a reduced fraction of immunoglobulins or totally free of immunoglobulins, characterized by further steps of affinity chromatography and immunoaffinity.
