A process for the preparation of a concentrate of von Willebrand factor (FVW) by means of chromatography, and the concentrate of FVW and may therefore be obtained
LABORATOIRE FRANCAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES
发明人:
MARTEL, SERGE,CHTOUROU, SAMI,POULLE, MICHEL
申请号：
DK05291553
公开号：
DK1632501T3
申请日:
2005.07.20
申请国别(地区):
DK
年份:
2014
代理人:
摘要：
Preparation of a concentrate (I) with von Willebrand factor (high purity) from a biological fraction containing von Willebrand factor, comprises a anion exchange chromatographic separation of vinyl polymer support (weak base type). Preparation of a concentrate with von Willebrand factor of high purity from a biological fraction containing von Willebrand factor, comprises a anion exchange chromatographic separation of vinyl polymer support (weak base type), where the separation comprises: (a) charging the chromatographic support with the fraction containing von Willebrand factor, previously balanced with an appropriate buffer at a predetermined flow, which allows the retention of the von Willebrand factor； (b) washing the support by an acid buffer with a flow higher than step (a) until elimination of proteins and non-retained contaminants； (c) rinsing and balancing the chromatographic support with the buffer and the flow of step (a)； and (d) eluting the von Willebrand factor by increasing the ionic force of the buffer. ACTIVITY : Coagulant. MECHANISM OF ACTION : None given.
