processes for obtaining biflavonoids, photoprotective, antioxidant, anti-inflammatory and anti-aging activities and their use in pharmaceuticals, nutraceuticals and cosmetics.
UNIVERSIDADE DE SÃO PAULO - USP;FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
发明人:
LYDIA FUMIKO YAMAGUCHI,MASSUO JORGE KATO,PAOLO DI MASCIO
申请号：
BR0305913
公开号：
BRPI0305913B1
申请日:
2003.12.12
申请国别(地区):
BR
年份:
2015
代理人:
摘要：
"Processes for obtaining biflavonoids, photoprotective, antioxidant, anti-inflammatory and anti-aging activities and their use in pharmaceuticals, nutraceuticals and cosmetics." The present invention relates to processes for obtaining a biflavonoid fraction from araucaria angustifolia leaves, photoprotective, antioxidant, anti-inflammatory and anti-aging activities and their use in the manufacture of cosmetics and medicaments useful in photoprotection, antioxidants, anti-inflammatory and anti-aging. More specifically, one of the processes for obtaining the biflavonoid fraction comprises the following steps: a) collecting fresh parts of the araucaria angustifolia plant； (b) drying the parts of the angustifolia plant in a greenhouse at a temperature ranging from 30 to 70 ° C； c) milling the dried plant in a tiger knife mill to a particle size of 2 to 6 mesh； d) macerating the obtained powder at a temperature between 30 and 70 ° C with ethanol or any solvent of similar polarities for 2 to 6 days； e) preparing the extract by filtering the solution obtained by macerating with filter paper followed by concentration under reduced pressure at a temperature of 40 <；sym>； 20 <；198>； c； down to 1/3 of the initial volume； f) precipitating the biflavonoid fraction by adding 1 to 3 l of water to the extract, followed by evaporation at a temperature between 30 and 70 ° C, at reduced pressure to remove the solvent； g) centrifuging or decanting the resulting precipitate to separate the biflavonoid fraction from the supernatant, followed by drying the solid obtained at 25 <；sym>； 20 <；198>； c； h) characterization of the bifiavonoid fraction by high performance liquid chromatography, high performance liquid chromatography coupled with mass spectrometry, planar chromatography and nuclear magnetic resonance； i) identification of bifiavonoid fraction components using established standards as a comparison."processos de obtenção de biflavonóides, atividades fotoprotetora, a
