Tomasz Lipinski,Andrzej Gamian,Ewa Zuziak,Agnieszka Korzeniowska-Kowal,Andrzej Gorski
申请号：
US12449418
公开号：
US09255251B2
申请日:
2008.02.08
申请国别(地区):
US
年份:
2016
代理人:
摘要：
A method of preparation of purified bacteriophage with increased antibacterial activity, in which from bacterial lysate of phages is obtained, advantageously in the presence of lysozyme, chelating factor and detergent, in a continuous manner with ultrafiltration on membranes, the phage containing high molecular mass preparation, devoid of bacterial cell wall and other contaminants, free of toxins and endotoxins, active in tests of bacterial lysis, which is characterized by chromatography HPLC, In SDS-PAGE electrophoresis, immunoblotting, biological tests of bacterial lysis, and is dedicated for phage therapy of bacterial infections and tumors and for production of phage deriving pharmaceutical preparations.
