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Preparativni postopek čiščenja humanega furina
专利权人:
BAXTER INTERNATIONAL INC.;BAXTER HEALTHCARE S.A.
发明人:
MATTHIESSEN PETER,ROMEDER-FINGER STEFAN,TURECEK PETER,SCHWARZ HANS-PETER,Matthiessen Peter,Romeder-Finger Stefan,Turecek Peter,Schwarz Hans-Peter
申请号:
SI200830942
公开号:
SI2157177T1
申请日:
2008.05.21
申请国别(地区):
SI
年份:
2013
代理人:
摘要:
Recombinant truncated human furin was expressed in CHO cells and concentrated approximately 50 -fold by ultrafiltration and diafiltration. The concentrate was purified by column chromatography on Capto-MMC„¢ resulting in a 30-50 fold purification factor and a yield of at least 60%. The at least 20% pure preparation obtained after Capto-MMC„¢ chromatography had already a purification degree allowing on-column maturation of pro-VWF. Then an additional Arginine Sepharose chromatography purification was carried out. This two column process for purification of truncated human furin resulted in an almost pure furin preparation with a specific activity of approximately 290,000 U furin/mg protein and a yield of about 50%.
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