Romeder-Finger, Stefan,Schwarz, Hans-Peter,Turecek, Peter,Matthiessen, Peter
申请号：
AU2008253230
公开号：
AU2008253230B2
申请日:
2008.05.21
申请国别(地区):
AU
年份:
2013
代理人:
摘要：
Recombinant truncated human furin was expressed in CHO cells and concentratedapproximately 50 -fold by ultrafiltration and diafiltration. The concentratewas purified by column chromatography on Capto-MMC™ resulting in a 30-50fold purification factor and a yield of at least 60%. The at least 20% pure preparationobtained after Capto-MMC™ chromatography had already a purificationdegree allowing on-column maturation of pro-VWF. Then an additional ArginineSepharose chromatography purification was carried out. This two column processfor purification of truncated human furin resulted in an almost pure furin preparationwith a specific activity of approximately 290,000 U furin/mg protein and a yieldof about 50%.
