A particle for detecting and analyzing a bio material, composition containing the same and manufacturing method are provided to enhance the binding property of particle with bio material and have particle which is mechanically and chemically stable. A method for manufacturing the composition having a particle for detecting and analyzing a bio material comprises: a step of mixing the bio material having a biotin with a nano bead which is coated with streptavidin and combining the biotin and streptavidin (S210)； a step of mixing with a hydrogel (S220)； a step of combining the biomaterial(130) having biotin(131) with nano bead(120) in which streptavidin(121) is coated； and a step of adding the hydrogel(110) to produce a bio complex particle composition(201).본 발명은 생체물질 검출 또는 분석용 입자, 이것을 포함하는 조성물 및 이것의 제조방법에 대한 것으로, 하이드로겔 내부 또는 표면에 스트렙타비딘(streptavidin)이 코팅된 나노비드를 포함하는 생체물질 검출 또는 분석용 입자에 의하여, 비오틴(biotin)을 가지는 생체물질과의 결합성을 높일 수 있는 것이다.
