SAMUILENKO, Anatoly Yakovlevich,САМУЙЛЕНКО, Анатолий Яковлевич,MASLOV, Evgeny Vitalyevich,МАСЛОВ, Евгений Витальевич,KHAIKINA, Lyudmila Sergeevna,ХАЙКИНА, Людмила Сергеевна,IVANOVA, Aleksandra Fedorov
申请号：
RURU2014/000149
公开号：
WO2014/168510A1
申请日:
2014.03.06
申请国别(地区):
WO
年份:
2014
代理人:
摘要：
The invention relates to veterinary biotechnology, in particular, culturing animal cells and viruses, and can be used for optimising reproduction of viruses in a cell culture and for producing medicines for a specific immunoprotection against rabies. In order to improve the quality of the final product by increasing the yield of a viral antigen having an enhanced immunogenic activity, as well as to simplify the manufacturing technology of the rabies vaccine, in the method for producing rabies vaccine comprising cultivating BHK-21 transplantable cell culture, infecting the cells with rabies virus, replicating the virus, and inactivating the virus-containing suspension with ß-propiolactone followed by obtaining the final product, the cultivation of BHK-21 transplantable cell culture is performed for 48-72 hours to a concentration of (2.5-3.0)106 cells/ml followed by infecting the cells with rabies virus in a dose of 0.01-0.1 mMLD50/cell, maintaining at a temperature of 38.5-39.5°C for 60-90 minutes followed by diluting with a growth medium to a concentration of a cell sub-line of (0.5-0.6)106 cells/ml, and continuing the cultivation of the resulting suspension for 48-72 hours at a temperature of 37-38°C and a pH value of 7.1-7.3 subsequently, the virus-containing suspension is cooled to a temperature of 4-8°C at pH 7.8-8.0, and tris(hydroxymethyl)aminomethane and ethylenediaminotetraacetic acid disodium salt are added.Linvention concerne la biotechnologie vétérinaire et notamment la culture des cellules danimaux et de virus et peut sutiliser pour optimiser la reproduction des virus dans la culture des cellules et obtenir des médicaments pour la prévention immunitaire spécifique de la rage. Pour améliorer la qualité du produit cible grâce à une production plus importante dantigène viral possédant une activité immunogène plus intense et pour simplifier la technologie industrielle de fabrication du vaccin antirabique, ce procédé de production de vaccin antirabique compre
