A kind of composition and method, it is in vitro for activating immune cell or induce in subject the composition of immune response, include at least one chiral cation lipid. In one embodiment, including chiral cation lipid non-steroidal cation lipid, there is the structure indicated by Formulas I) Wherein R1 is quaternary ammonium group, and Y1 is interval base, hydrocarbon chain of choosing from, ester, ketone and peptide, and C* is chiral carbon, and R2 independently is saturated fatty acid of choosing from, unsaturated fatty acid, a kind of hydrocarbon that ester connects with R3, phosphorus diester, and combinations thereof.
