SALVADOR PEIR,MARA EUGENIA CASTELLI,LEANDRO VETCHER,HUGO GABRIEL MENZELLA
申请号：
ARP140102479
公开号：
AR096800A1
申请日:
2014.07.03
申请国别(地区):
AR
年份:
2016
代理人:
摘要：
A modified Enzyme phospholipase C from Bacillus cereus, as well as a method to use the modified Enzyme in a Treatment method of vegetable Oil.In some embodiments, the method may include combining Vegetable Oil with phospholipase C, an enzyme modified comprising an Amino Acid Sequence present at least 85% Homology to the sequence of SEQ ID no. 1, where the amino acid residue at position 66 is TRP (W) or (and), TyrAnd maintaining the Reaction mixture under appropriate conditions for this Enzyme catalyzes the hydrolysis of phospholipids in oil to produce diacylglycerol and soluble phosphate in water.Claim 11: Composition characterized in that it comprises: a Vegetable Oil and a phospholipase C Protein whose Amino Acid Sequence has a Homology of at least 85% at the amino acid sequence of SEQ ID no. 1, where the amino acid residue at position 66 is Selection Ado the set comprised TRP Tyr (W) and (y).Claim 21: a Nucleic Acid encoding the Protein of Claims 17. Claim: The 24 cell of claim 23, wherein the cell is a cell of Pichia pastoris Nucleotide sequence comprising the said Nucleic Acid SEQ ID no. 2 and codon positions defined by 196 - 198 is selected from the whole period By TGG and CT.Una enzima fosfolipasa C de Bacillus cereus modificada, así como también un método para usar dicha enzima modificada en un método de tratamiento de aceite vegetal. En algunas realizaciones este método puede comprender combinar un aceite vegetal con una enzima fosfolipasa C modificada que comprenda una secuencia de aminoácidos que presente una homología de al menos 85% a la secuencia de SEQ ID Nº 1, donde el residuo de aminoácido en la posición 66 es Trp (W) o Tyr (Y), y mantener la mezcla de reacción en condiciones apropiadas para que dicha enzima catalice la hidrólisis de fosfolípidos en el aceite para producir diacilglicerol y fosfato soluble en agua. Reivindicación 11: Una composición caracterizada porque comprende: aceite vegetal y una proteína fosfolipasa C cuya la secuencia de amino
