A culture medium of Antrodia camphorate mycelium and an Antrodia camphorate mycelium culture method using said culture medium are disclosed. The culture medium includes powdered grape fruit peel, powdered buckwheat and pure water. The weight percentage of the powdered grape fruit peel can be 1 to 17, the weight percentage of the powdered buckwheat can be 30 to 69, and the weight percentage of the pure water can be 30 to 60. Furthermore, the powdered grape fruit peel is provided to increase expression levels of crude triterpene of Antrodia camphorate mycelium. The Antrodia camphorate mycelium culture method using said culture medium includes steps of providing an Antrodia camphorate strain, and inoculating said Antrodia camphorate strain to said culture medium which includes said powdered grape fruit, said powdered buckwheat, and said pure water.本發明揭露一種牛樟芝菌絲體發酵培養基及應用此牛樟芝發酵培養基的培養方法。其中,牛樟芝菌絲體發酵培養基包含葡萄柚果皮烘乾粉末、蕎麥粉末以及純水。葡萄柚果皮烘乾粉末之重量百分比可為1至17,蕎麥粉末之重量百分比可為30至69,而純水之重量百分比可為30至60。更進一步地說,葡萄柚果皮烘乾粉末係用以作為牛樟芝的粗三萜表現量之誘導劑。應用牛樟芝菌絲體發酵培養基的培養方法包含先提供一牛樟芝種菌,再將該牛樟芝種菌接種至前述包含葡萄柚果皮烘乾粉末、蕎麥粉末以及純水之牛樟芝發酵培養基中進行發酵培養以生成牛樟芝菌絲體。100‧‧‧步驟200‧‧‧步驟
