PROBLEM TO BE SOLVED: To provide a plant viral vector for expression of a foreign gene in a plant, which can be conveniently removed after completion of genome editing and can minimize its adverse effects of the removal on the plant and to provide a method for gene introduction, the method enabling genome editing without incorporating foreign DNA into a plant chromosome, the method comprising a step of transmitting the plant viral vector to the plant.SOLUTION: According to the present invention, there is provided a plant viral vector for genome-editing enzyme gene transfer, having a miR398 target nucleic acid sequence targeted by miR398 comprising a specific nucleic acid sequence. There is also provided a viral vector in which the miR398 target nucleic acid sequence is complementary to a nucleic acid sequence having at least 70% identity to miR398.SELECTED DRAWING: NoneCOPYRIGHT: (C)2018,JPO&INPIT【課題】植物における外来遺伝子の発現のために、ゲノム編集完了後にウイルスベクターを簡便に除去可能であり、且つ前記除去操作による前記植物への悪影響を最小限にできる前記植物ウイルスベクターと、前記植物ウイルスベクターを前記植物に感染させる工程を含みながら、外来DNAを植物染色体に組み込むことなくゲノム編集を可能とする遺伝子導入方法の提供。【解決手段】特定の核酸配列を含むmiR398により標的とされるmiR398標的核酸配列を有する、ゲノム編集酵素遺伝子導入のための植物ウイルスベクター。miR398標的核酸配列が、miR398と少なくとも70%の同一性を有する核酸配列に相補的であるウイルスベクター。【選択図】なし
