Disclosed is a method for preparation of exosomes having a hair regeneration effect, the method comprising the steps of: providing direct or indirect ultrasonic stimuli for cells； incubating a mixture of the cells and a medium for a predetermined period of time； and separating exosomes from the mixture, wherein the providing of direct ultrasonic stimuli refers to applying ultrasonic stimuli to a medium containing cells and the providing of indirect ultrasonic stimuli refers to applying ultrasonic stimuli to a cell-free medium which is then mixed with the cell. Such a method for preparation of exosomes allows the acquirement of exosomes having a hair regeneration effect at high yield within a short period of time through the simple process of ultrasonication from stem cells difficult to handle as well as somatic cells that can be easily obtained and maintained. The exosomes thus obtained also contain a large quantity of various factors. The exosomes and a composition containing same have increased numbers of various factors capable of inducing hair regeneration. Having a phospholipid-based membrane structure, the exosomes can easily penetrate into the scalp and deliver a material at a high rate to effectively induce hair regeneration. The exosomes are advantageously free of the adverse effects resulting from the use of synthetic medications.L'invention concerne un procédé de préparation d'exosomes possédant un effet de régénération sur les cheveux, le procédé comprenant les étapes consistant : à fournir des stimuli ultrasonores directs ou indirects destinés aux cellules ； à incuber un mélange comprenant les cellules et un milieu pendant une période de temps prédéterminée ； et à séparer les exosomes du mélange, la fourniture de stimuli ultrasonores directs se rapportant à l'application de stimuli ultrasonores à un milieu contenant des cellules, et la fourniture de stimuli ultrasonores indirects se rapportant à l'application de stimuli ultrasonores à un milieu exempt d
