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Variants with improved Properties of chymosin - S1 - casein
专利权人:
CHR. HANSEN A/S
发明人:
LONE POULSEN,PIA RASMUSSEN,TINA HORNBAEK,CECILIE LYKKE MARVIG NIELSEN
申请号:
ARP160102650
公开号:
AR105867A1
申请日:
2016.08.31
申请国别(地区):
AR
年份:
2017
代理人:
摘要:
Claim 1: A variant of chymosin polypeptide isolated, characterized in that: (a) the isolated polypeptide variant of chymosin C / p has a value that is at least 200% of C / p value of chymosin Camel isolated characterized by the mature polypeptide of SEQ ID no. 2; and (b) Variant of chymosin Cleaves the polypeptide isolated AS1 casein - with a Frequency lower than 80%The frequency of excision of AS1 casein to chymosin - camel isolated characterized by the mature polypeptide of SEQ ID no. 2, where the Division of AS1 - casein was determined by Quantification of peptides obtained by Incubation of AS1 casein and skim milk with variant of Q Uimosina or chymosin CamelWhere the quantification is performed by rp-hplc coupled with a Mass Spectrometer ESI q-tof.Claim 9: a method for preparing a variant of chymosin polypeptide isolated according to any one of claims 1 to 4, which comprises the following steps: (a) preparation of an alteration in one or several positions in the DNA Sequence that encodes the polip u00e9pt Gradually Mature of SEQ ID no. 2, where the alteration involves a substitutionA deletion or Insertion in at least one amino acid position; (b): Production and isolation of altered polypeptide of Step (a). Claim 16: use a variant of polypeptide of chymosin according to claim 15 in a process for preparing pasta filata Cheese, Cheddar, Continental type Soft Cheese or Salted White Cheese.Reivindicación 1: Una variante de polipéptido de quimosina aislada caracterizada porque: (a) la variante de polipéptido de quimosina aislada tiene un valor C/P que es al menos el 200% del valor C/P de quimosina de camello aislada caracterizada por el polipéptido maduro de SEQ ID Nº 2; y (b) la variante de polipéptido de quimosina aislada escinde la aS1-caseína con una frecuencia inferior al 80% de la frecuencia de escisión de aS1-caseína de quimosina de camello aislada caracterizada por el polipéptido maduro de SEQ ID Nº 2, en donde la escisión de aS1-caseína se determina por cua
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