To provide, for example, methods for producing retinal tissues and retinal-related cells such as retinal progenitor cells and retinal layer-specific nerve cells from pluripotent stem cells.SOLUTION: The present invention discloses, for example, a method for producing retinal progenitor cells, the method comprising a first step of suspending and culturing pluripotent stem cells in a serum-free medium to form an aggregate of the pluripotent stem cells, and a second step of suspending and culturing the formed aggregate in a serum-free medium or serum medium containing a BMP signaling pathway active substance without a sonic hedgehog signaling pathway active substance to obtain an aggregate containing retinal progenitor cells.SELECTED DRAWING: None【課題】網膜組織、並びに網膜前駆細胞及び網膜層特異的神経細胞等の網膜関連細胞を多能性幹細胞から製造する方法等を提供する。【解決手段】多能性幹細胞を無血清培地中で浮遊培養することにより多能性幹細胞の凝集体を形成させる第一工程、及び、形成された凝集体を、ソニック・ヘッジホッグシグナル伝達経路作用物質を含まずBMPシグナル伝達経路作用物質を含む無血清培地又は血清培地中で浮遊培養し、網膜前駆細胞を含む凝集体を得る第二工程を含む、網膜前駆細胞の製造方法等を開示する。【選択図】なし
