The present invention includes (1) a first step in which pluripotent stem cells are suspended in a serum-free medium to form an aggregate of pluripotent stem cells, and (2) an aggregate formed in step (1). A retina comprising a second step of obtaining an aggregate containing retinal progenitor cells by suspension culture in a serum-free medium or serum medium containing a BMP signaling pathway agent but not containing a sonic hedgehog signaling pathway agent A method for producing progenitor cells is provided.本発明は、（１）多能性幹細胞を無血清培地中で浮遊培養することにより多能性幹細胞の凝集体を形成させる第一工程、及び（２）工程（１）で形成された凝集体を、ソニック・ヘッジホッグシグナル伝達経路作用物質を含まずＢＭＰシグナル伝達経路作用物質を含む無血清培地又は血清培地中で浮遊培養し、網膜前駆細胞を含む凝集体を得る第二工程を含む、網膜前駆細胞の製造方法を提供する。
