The present invention provides a method for producing ciliary marginal stem cells that are induced to differentiate from pluripotent stem cells, including either or both of the following steps (1) and (2): (1) A step of suspending and culturing cells obtained from cell aggregates including ciliary marginal-like structures derived from pluripotent stem cells to obtain retinosphere； and (2) differentiation induced from pluripotent stem cells. A step of sorting stage specific embryonic antigen-1-positive cells from cells obtained from cell aggregates containing a ciliary marginal-like structure. According to the present invention, ciliary peripheral edge stem cells having the ability to differentiate into retinal cells including retinal layer-specific neurons and self-replicating ability can be efficiently produced with high purity.本発明は、以下の工程（１）もしくは工程（２）のいずれか又はこれら両工程を含む、多能性幹細胞から分化誘導された毛様体周縁部幹細胞の製造方法を提供する：（１）多能性幹細胞から分化誘導された毛様体周縁部様構造体を含む細胞凝集体から得られた細胞を浮遊増殖培養し、レチノスフェアを得る工程；及び（２）多能性幹細胞から分化誘導された毛様体周縁部様構造体を含む細胞凝集体から得られた細胞からstage specific embryonic antigen-1陽性細胞を分取する工程。 本発明により、網膜層特異的神経細胞を含む網膜細胞への分化能及び自己複製能を有する毛様体周縁部幹細胞を高純度に効率的に製造することが可能となる。
