PROBLEM TO BE SOLVED: To provide muscle-derived progenitor cells that show long-term survival following transplantation into body tissues and which can augment soft tissue following introduction (e.g., via injection, transplantation, or implantation) into a site of soft tissue, and to provide methods for isolating muscle-derived progenitor cells.SOLUTION: The method for treating a urinary tract disease includes: a step of isolating skeletal muscle cells from a human a step of cooling the cells to a temperature lower than 10°C and storing the cells for 1-7 days a step of suspending the human skeletal muscle cells in a first cell culture container between 30 and 120 minutes and decanting the media to a second cell culture container a step of allowing the remaining cells in the media to attach to the walls of the second cell culture container and a step of isolating the cells from th walls of the second cell culture container, wherein the isolated cells are muscle derived progenitor cells (MDCs).COPYRIGHT: (C)2013,JPO&INPIT【課題】体組織への移植後に長期生存を示しかつ軟組織の部位への導入(例えば、注射、移植もしくは埋め込みを介して)後に軟組織を増大させ得る筋肉由来前駆細胞の提供、および筋肉由来前駆細胞を単離するための方法の提供。【解決手段】ヒトから骨格筋細胞を単離し;10℃より低い温度に冷却し、1~7日間保存する工程;第1の細胞培養容器中で30~120分間懸濁し、第2の細胞培養容器へと培地を移す工程;該培地中に残っている細胞を、該第2の細胞培養容器の壁に付着させる工程;該第2の細胞培養容器の壁から該細胞を単離する工程であって、ここで該単離される細胞は、筋由来前駆細胞(MDC)である。【選択図】図1
