The purpose of the present invention is to provide a sheet shaped cell culture that is highly active with respect to cytokine production ability and the like and a production method for the sheet shaped cell culture. The present invention relates to: a production method for a sheet shaped cell culture that includes a step in which cells are frozen a step in which the frozen cells are thawed and a step in which a sheet shaped cell culture is formed a sheet shaped cell culture that is produced using the production method and that is highly active and a method in which the sheet shaped cell culture is used to treat a disease that is associated with tissue abnormality.
