This invention provides a membrane structure of a deccellularized tissue matrix layer containing matrix fiber in nanometer scale. Such nano-fiber’s width in the matrix subsequent to lyophilization is ranging from 50 nm to 500 nm. The membrane structure is further covered by an artificial layer of collagen or materials derived from collagen, resulting in a multilayered membrane.本發明揭示一種去細胞化之組織膜結構,其中該細胞化之組織膜結構係含有奈米級細胞間質纖維,且該奈米級細胞間質纖維之寬度於膜進行冷凍乾燥後係界於50 nm~500 nm。該去細胞化組織膜結其進一步與一膠原蛋白或其衍生物之人工層狀結構結合成一多層狀膜結構。102‧‧‧細胞間質纖維
