method for evaluating an agent acting on a gene product, method for evaluating whether a gene product is a suitable target for drug discovery, and method for evaluating whether a gene product is a suitable target for evaluating an agent's ability to act on gene product
MASSACHUSETTS INSTITUTE OF TECHNOLOGY;MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V.;UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER;WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
发明人:
DAVID P. BARTEL,PHILLIP A. SHARP,PHILLIP D. ZAMORE,THOMAS TUSCHL
申请号：
BR0117341
公开号：
BRPI0117341B1
申请日:
2001.03.30
申请国别(地区):
BR
年份:
2016
代理人:
摘要：
Method for Evaluating Whether an Agent Acts on a Gene Product and Method for Evaluating Whether a Gene Product is a Suitable Target for Drug Discovery The present invention relates to an in vitrode drosophila system that has been used to demonstrate that dsrna is processed for 21-23 nucleotide length rna segments. In addition, when these 21-23 nt fragments are purified and added back to the drosophila extracts, they mediate rna interference in the absence of long dsrna. therefore, these 21-23 nt fragments are the specific mediators for rna degradation sequence. A molecular signal, which may be its specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNA. This invention encompasses these 21-23 nt fragments and their use to specifically inactivate gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long mRNAs to produce gene function is useful in genomic and functional therapeutic applications.método para avaliar se um agente age em um produto de gene e método para avaliar se um produto de gene é um alvo adequado para descoberta de droga a presente invenção relaciona-se com um sistema in vitrode drosophila que foi usado para demonstrar que dsrna é processado para segmentos de rna de 21-23 nucleotídeos de comprimento. além disso, quando estes fragmentos de 21-23 nt são purificados e adicionados de volta aos extratos de drosophila, eles medeiam interferência de rna na ausênciade dsrna longo. portanto, estes fragmentos de 21-23 nt são os mediadores específicos para seqúêncía de degradação de rna. um sinal molecular, que pode ser seu comprimento específico, deve estar presente nestes fragmentos de 21-23 nt para recrutar fatores celulares envolvidos em rnaí. esta presente invenção abrange estes fragmentos de 21-23 nt e seu uso para inativar especificamente função de gene
