Gellan crowds hydrogel discloses the application of Cell culture invitro and organizational project and regenerative medicine. This gellan crowd hydrogel can be used alone, or with living cells and/or biomolecule to people and/or animal. The gellan gum of chemical modification and selected ion-chelating fiber substituent group provide novel gellan gum hydrogel with tunable physical chemistry and biological property. It is more than existing hydrogel system that advantageous as described herein, which is presented, in the modified gellan gum hydrogel, including solubility, ionic crosslinking versatility, convenient for prepare and syringeability and bigger adhesiveness in biological tissue and surface, keeping the cell of encapsulating simultaneously is feasible, expression and up-regulation healthy cell epimatrix label in long culture period.
