Beihao Stem Cell and Regenerative Medicine Research Institutes Co., Ltd.;Peking University
发明人:
Hongkui DENG,Xiang LI,Xiaohan ZUO
申请号：
US15741204
公开号：
US20180193386A1
申请日:
2016.07.01
申请国别(地区):
US
年份:
2018
代理人:
摘要：
Cocktails of chemical inducers of neuron-like properties (CINP) is provided, which includes cAMP agonists, neurogenic small molecules, glycogen synthase kinase inhibitors, TGFβ receptor inhibitors, and BET family bromodomain inhibitors and optionally, a selective inhibitor of ROCK or p38 MAPK. These cocktails are used in a method of inducing neuron-like properties in partially or completely differentiated non-neuronal cells. The method includes contacting cells of a first type (non-neuronal) with the CINPs for a sufficient period of time to result in reprogramming the cell into cells of a second type having neuron-like characteristics (CiNs). Isolated chemically induced neurons (CiNs) can be used in a number of applications, including but not limited to cell therapy.
