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[学术文献 ] Metabolite Profiling and Quantitation of Cucurbitacins in Cucurbitaceae Plants by Liquid Chromatography coupled to Tandem Mass Spectrometry（采用液相色谱-串联质谱法对葫芦科植物葫芦素的代谢产物进行分析和定量） 进入全文
Springer Nature Journal
Cucurbitaceae is an important plant family because many of its species are consumed as food, and used in herbal medicines, cosmetics, etc. It comprises annual vines and is rich in various bioactive principles which include the cucurbitacins. These steroidal natural products, derived from the triterpene cucurbitane, are mainly the bitter principles of the family Cucurbitaceae. Their biological activities include anti-inflammatory, hepatoprotective, and anti-cancer activities. A total of 10 species belonging to 6 genera of the Cucurbitaceae family along with Cissampelos pareira (Menispermaceae) were included in this study. A comprehensive profiling of certain natural products was developed using HPLC-QTOF-MS/MS analysis and a distribution profile of several major natural products in this family was obtained. A total of 51 natural products were detected in both positive and negative ionization modes, based on accurate masses and fragmentation patterns. Along with this, quantitation of four bioactive cucurbitacins, found in various important plants of the Cucurbitaceae family, was carried out using multiple reaction monitoring (MRM) approach on an ion trap mass spectrometer. Cucurbitacin Q was found to be the most abundant in C. pareira , while Citrullus colocynthis contained all four cucurbitacins in abundant quantities. The developed quantitation method is simple, rapid, and reproducible.
[学术文献 ] Characterization of VQ motif-containing protein family and their expression patterns under phytohormones and abiotic stresses in melon ( Cucumis melo L.)（甜瓜( 瓜蒂 ) VQ motif蛋白家族的特征及其在植物激素和非生物胁迫下的表达模式。） 进入全文
Springer Nature Journal
Many studies have showed plant VQ gene family plays an important part in response to abiotic and biotic stresses. At present, VQ protein family has been found successively in many plants, however, there have no detailed characterization and expression profiles of VQ gene family in muskmelon. Thus, the author probed into genome-wide identification, characterization and expression pattern under various hormones and abiotic stresses. Thirty CmVQ genes were classified ten groups according to orthologous of Arabidopsis VQ genes and dispersedly distributed on 11 chromosomes except for chromosomes 5 and 9. Majority of CmVQ members contained 300 amino acids or less, and were predicted to localize in the nucleus and had no intron. Many cis -elements in response to different stresses and hormones were found in the promoters of CmVQ genes. Gene ontology analysis displayed that 60% CmVQ genes participated in response to stimuli. Quantitative real-time PCR displayed that numerous CmVQ genes showed either positively or negatively regulated expression patterns under hormonal treatments and abiotic stresses. In summary, this research offers a general introduction to melon VQ genes and will contribute to the molecular breeding for resistance to stresses, as well as deeper inquiry on the biological functions of the VQ proteins.
[相关专利 ] 一种水稻种子背侧糊粉层特异表达的启动子P-MYBLIKE02 及其应用 进入全文
佰腾网
本发明公开了一种水稻种子背侧糊粉层特异表达的启动子P-MYBLIKE02 及其应用。本发明的启动子P-MYBLIKE02 为如下1)-3) 中任一所述的DNA 分子：1) 序列表的序列1 所示的DNA 分子；2)在严格条件下与1) 所述DNA 分子杂交且具有启动子功能的DNA 分子；3) 与1) 中所述DNA 分子具有90％以上同源性，且具有启动子功能的DNA 分子。利用本发明的水稻背侧糊粉层中特异表达的启动子可以驱动目的基因的表达，该启动子不仅对于研究糊粉层的发育调控和生产应用具有重要的意义，而且将为提高水稻种子蛋白质、脂肪酸、铁、锌、维生素B1 等营养成分的含量提供理论指导。
[相关专利 ] 一种水稻种子背侧糊粉层特异表达的启动子P-C2H201 及其应用 进入全文
佰腾网
本发明公开了一种水稻种子背侧糊粉层特异表达的启动子P-C2H201 及其应用。本发明的启动子P-C2H201 为如下1)-3) 中任一所述的DNA 分子：1) 序列表的序列1 所示的DNA 分子；2) 在严 格条件下与1) 所述DNA 分子杂交且具有启动子功能的DNA 分子；3) 与1) 中所述DNA 分子具有90％以上同源性，且具有启动子功能的DNA 分子。利用本发明的水稻背侧糊粉层中特异表达的启动子可以驱动目的基因的表达，该启动子不仅对于研究糊粉层的发育调控和生产应用具有重要的意义，而且将为提高水稻种子蛋白质、脂肪酸、铁、锌、维生素B1 等营养成分的含量提供理论指导。
[相关专利 ] 与植物抗逆性相关的蛋白BhDNAJC2及其编码基因与应用 进入全文
佰腾网
本发明公开了一种与植物抗逆性相关的蛋白BhDNAJC2及其编码基因与应用。本发明所提供的蛋白是如下a )或b )：a )由序列表中序列1所示的氨基酸序列组成的蛋白质；b)将a )所限定的蛋白质的氨基酸序列经过一个或几个氨基酸残基的取代和/或缺失和/或添加，且与植物抗逆性相关的蛋白质。实验证明，将蛋白的编码基因导入野生型拟南芥，得到转基因拟南芥，与野生型拟南芥相，抗旱、抗盐和抗碱性明显提高。本发明对于培育抗旱、抗盐和抗碱性提高的作物、林草等新品种具有重要的理论及实际意义，可用于农牧业和生态环境治理所需的抗性植物品种的培育与鉴定。
[相关专利 ] SSR 基因在调控植物根生长发育中的应用 进入全文
佰腾网
本发明公开了一种SSR 基因在调控植物根生长发育和生长素运输中的应用。本发明公开一种抑制植物的根生长的方法，是敲除植物中的SSR基因。本发明证明拟南芥SSR 基因在植物的根生长及生长素运输中发挥了重要作用。
