UNIVERSITY OF MARYLAND, BALTIMORE;GPB SCIENTIFIC, LLC;THE TRUSTEES OF PRINCETON UNIVERSITY
发明人:
WARD, ANTHONY,CAMPOS-GONZALEZ, ROBERTO,SKELLEY, ALISON,GANDHI, KHUSHROO,GRISHAM, MICHAEL,CIVIN, CURT,STURM, JAMES C.
申请号：
CA3074495
公开号：
CA3074495A1
申请日:
2018.08.22
申请国别(地区):
CA
年份:
2019
代理人:
摘要：
The present invention is directed to the use of microfluidics in the preparation of cells and compositions for therapeutic uses. In its first aspect, the invention is directed to a method of genetically engineering a population of target cells. This is done by isolating the target cells from a crude fluid composition by performing Deterministic Lateral Displacement (DLD) on a microfluidic device. The invention includes a method of producing CAR T cells by obtaining a crude fluid composition comprising T cells and performing DLD on the composition using a microfluidic device. In another aspect, the invention is directed to a method of treating a patient for cancer, an autoimmune disease or an infectious disease by administering CAR T cells engineered according to the method.
