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Data from: Comparative transcriptomics and gene expression in larval tiger salamander (Ambystoma tigrinum) gill and lung tissues as revealed by pyrosequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.69t48k87
- 摘要：
- s. We do so with a hierarchical experimental design that captures variation in expression among genes, among tissues, and among individuals. Using 454 pyrosequencing, we produced
Data from: Transcriptome resources for the white-footed mouse (Peromyscus leucopus): new genomic tools for investigating ecologically divergent urban and rural populations
- 负责人：
- DOI：
- doi:10.5061/dryad.6hc0f
- 摘要：
- two Next Generation Sequencing (NGS) platforms, 454 and SOLiD 5500XL, to assemble low coverage transcriptomes of the white-footed mouse (Peromyscus leucopus), a widespread and abundant
Data from: Accelerated evolutionary rate of housekeeping genes in tunicates
- 负责人：
- DOI：
- doi:10.5061/dryad.8323
- 摘要：
- inly lies in studies focusing on only a few model species. In order to expand the available genomic data for the group, we used the high-throughput 454
Data from: Exploring Symbiodinium diversity and host specificity in Acropora corals from geographical extremes of Western Australia with 454 amplicon
- 负责人：
- DOI：
- doi:10.5061/dryad.7th55
- 摘要：
- contrasting regions of Western Australia. The application of 454 pyrosequencing allowed for detection of Symbiodinium operational taxonomic units (OTUs
Data from: De novo transcriptome characterization and development of genomic tools for Scabiosa columbaria L. using next-generation sequencing techniques.
- 负责人：
- DOI：
- doi:10.5061/dryad.8160
- 摘要：
- nt for ecological and conservation purposes. Illumina and 454 sequencing are among the most used NGS technologies and have been shown to produce optimal result
Data from: Vertical distribution of the soil microbiota along a successional gradient in a glacier forefield
- 负责人：
- DOI：
- doi:10.5061/dryad.gp302
- 摘要：
- the vertical distribution of microorganisms in newly developed soils after glacier retreat. We used 454-pyrosequencing to assess whether bacterial
Data from: Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate DNA barcode capture from single specimens
- 负责人：
- DOI：
- doi:10.5061/dryad.p25vn
- 摘要：
- to DNA barcoding PCR primers. We employ 454 pyrosequencing to recover full-length DNA barcodes of 190 specimens using 12.5% capacity of a 454 sequencing ru
Data from: Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays
- 负责人：
- DOI：
- doi:10.5061/dryad.1pq21
- 摘要：
- , using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mea
Data from: Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers.
- 负责人：
- Brelsford, Alan
- DOI：
- doi:10.5061/dryad.1hg75
- 摘要：
- High-fidelity “proofreading” polymerases are often used in library construction for next-generation sequencing projects, in an effort to minimize errors in the resulting sequence data. The increased template fidelity of these polymerases can come at the cost of reduced template specificity, and library preparation methods based on the AFLP technique may be particularly susceptible. Here, we compare AFLP profiles generated with standard Taq and two versions of a high-fidelity polymerase. We find that Taq produces fewer and brighter peaks than high-fidelity polymerase, suggesting that Taq performs better at selectively amplifying templates that exactly match the primer sequences. Because the higher accuracy of proofreading polymerases remains important for sequencing applications, we suggest that it may be more effective to use alternative library preparation methods.
Data from: A next-generation sequencing approach to river biomonitoring using benthic diatoms
- 负责人：
- DOI：
- doi:10.5061/dryad.3gm8c
- 摘要：
- . Next Generation Sequencing (NGS) can be used to study communities of microorganisms, so we carried out a test of the reliability of 454 pyrosequencing for estimating diatom
