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Data from: Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping
- 负责人：
- DOI：
- doi:10.5061/dryad.tk467n8
- 摘要：
- Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.
Data from: Within-host competition between Borrelia afzelii ospC strains in wild hosts as revealed by massively parallel amplicon sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.vf83f
- 摘要：
- a protocol for 454 amplicon sequencing of ospC, which allows both detection and quantification of each individual strain in an infection. Each host individual
Data from: Characterization of transcriptomes from sexual and asexual lineages of a New Zealand snail (Potamopyrgus antipodarum)
- 负责人：
- DOI：
- doi:10.5061/dryad.v72m2
- 摘要：
- . We used 454 sequencing of cDNA libraries to generate transcriptomes from two sexual and two asexual P. antipodarum lineages. A de novo assembly of 116.7 Mb
Data from: Seasonality and locality affect the diversity of Anopheles gambiae and Anopheles coluzzii midgut microbiota from Ghana.
- 负责人：
- DOI：
- doi:10.5061/dryad.v0cf2
- 摘要：
- . The midgut microbiota of adults that emerged from these collections was determined by 454-pyrosequencing of the 16S ribosomal DNA. We show that in bot
Data from: Changes in the bacterial microbiota in gut, blood, and lungs following acute LPS instillation into mice lungs
- 负责人：
- 关键词：
- lungs;LPS;microbiome
- DOI：
- doi:10.5061/dryad.7874p
- 摘要：
- of the bronchoalveolar lavage fluid, blood, and cecum were determined using 454 pyrotag sequencing and quantitative polymerase chain reaction (qPCR) at 4 through 168 hours post
Data from: Assembly of root-associated bacteria communities: interactions between abiotic and biotic factors
- 负责人：
- DOI：
- doi:10.5061/dryad.7535k
- 摘要：
- ng 454 pyrosequencing, we examined RAB communities from two dominant alpine tundra plants, Geum rossii and Deschampsia cespitosa, under control, N addition
Data from: Host species and environmental effects on bacterial communities associated with Drosophila in the laboratory and in the natural environment
- 负责人：
- DOI：
- doi:10.5061/dryad.5q1sb
- 摘要：
- s shaping them is limited. We used 454-based sequencing of a variable region of the bacterial 16S ribosomal RNA gene to characterize the bacterial communities
Baboon genome sequencing project and diversity panel
- 负责人：
- BGI
- DOI：
- doi:10.26036/cnphis0001258
- 摘要：
- draft sequence of the reference genome has been produced from an olive baboon (Papio anubis), using read data produced by the Sanger, 454 and Illumina platforms
Data from: De novo characterization of the Timema cristinae transcriptome facilitates marker discovery and inference of genetic divergence.
- 负责人：
- DOI：
- doi:10.5061/dryad.70mv450r
- 摘要：
- lion 454 sequencing reads that assembled into 84,937 contigs representing approximately 18,282 unique genes and tens of thousands of potential
Data from: Signatures of rapid evolution in urban and rural transcriptomes of white-footed mice (Peromyscus leucopus) in the New York metropolitan area
- 负责人：
- DOI：
- doi:10.5061/dryad.r8ns3
- 摘要：
- -coding regions for an exemplar ‘urban adapter’. We used Roche 454 GS FLX+ high throughput sequencing to derive transcriptomes from multiple tissues from
