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Data from: Intra-individual polymorphism in chloroplasts from NGS data: where does it come from and how to handle it?
负责人:
关键词:
GATK;SNP;NGS;heterozygote;Chloroplast;SAMtools
DOI:
doi:10.5061/dryad.31733
摘要:
Next generation sequencing allows access to a large quantity of genomic data. In plants, several studies used whole chloroplast genome sequences
Data from: Cost-effective enrichment hybridization capture of chloroplast genomes at deep multiplexing levels for popula
负责人:
关键词:
DNA probes long range PCR MiSeq next generation sequencing plastomes whole chloroplast sequencing
DOI:
doi:10.5061/dryad.t6b05
摘要:
the effectiveness of this protocol to retrieve 95% of the sequence of the whole chloroplast on 95 multiplexed individuals in a single MiSeq ru
Data from: Dated tribe-wide whole chloroplast genome phylogeny indicates recurrent hybridizations within Triticeae
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关键词:
DOI:
doi:10.5061/dryad.25743
摘要:
relationships and estimate divergence times of the taxa. We present the most comprehensive dated Triticeae chloroplast phylogeny and review previous hypothese
Data from: Whole-chloroplast analysis as an approach for fine-tuning the preservation of a highly charismatic but critically endangered speci
负责人:
关键词:
Wollemia nobilis;Araucariaceae;endangered species;rainforest tree;chloroplast genome;conservation genetics
DOI:
doi:10.5061/dryad.6908r
摘要:
ss the chloroplast using shotgun sequencing, bioinformatic extraction of chloroplast DNA and variant detection. Six chloroplast single nucleotide polymorphisms wer
Data from: Complete chloroplast genome of the genus Cymbidium: lights into the species identification, phylogenetic implications
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关键词:
DOI:
doi:10.5061/dryad.14214
摘要:
m the complete chloroplast genome can provide effective tools for species identification and phylogenetic resolution. Results: The complete nucleotide sequences
Data from: Species level phylogeny and polyploid relationships in Hordeum (Poaceae) inferred by next-generation sequencing and in-silico cloning
负责人:
关键词:
Multispecies coalescent;nuclear single-copy genes;in-silico cloning;polyploidy;Hordeum;phylogeny;Evolution;systematics
DOI:
doi:10.5061/dryad.fn2nt
摘要:
olyploidization, knowledge of parental relationships is essential. One chloroplast and 12 nuclear single-copy loci were amplified by polymerase chain reaction (PCR) in al
Data from: A long PCR based approach for DNA enrichment prior to next-generation sequencing for systematic studies
负责人:
关键词:
Lupinus leucophylla Douglas ex Lindl.;Abies fraseri (Pursh) Poir;Castilleja linariifolia Benth.;Castilleja covilleana L.F.Hend.;Castilleja elmeri Fernald;Crataegus columbiana Howell;Bartsia inaequalis Benth.;Abies amabilis Douglas ex J.Forbes;Castilleja ortegae Standl.;Abies grandis (Douglas ex D. Don) Lindley;Poa bulbosa L.;Nuphar polysepalum Engelm.;Castilleja pallescens Greenm.;Next-generation sequencing;Universal Chloroplast PCR Primers;Penstemon montanus Greene var idahoensis (Keck) Cronq.;Bromus tectorum L.;Long PCR;Castilleja applegatei Fernald;Capsella bursa-pastoris (L.) Medik.;Castilleja miniata Douglas ex Hook.;Salix scouleriana Barrat ex. Hook.;Chloroplast Enrichment;Alnus rhombifolia Nutt.;Balsamorhiza sagittata (Pursh) Nutt.;Castilleja lineariloba (Benth.) T.I.Chuang & Heckard;Plastome;Castilleja victoriae Fairbarns & J.M.Egger;Castilleja arvensis Cham. & Schltdl.;Lomatium dissectum (Nutt) Mathias & Constance;Castilleja virgata (Domb. ex Wedd.) Edwin;Balsamorhiza hookeri Nutt;Bartsia stricta (Kunth) Benth.;Polygonum douglasii Greene;Castilleja oresbia Greenm.;Angiosperms;Lamourouxia virgata Kunth;Umbellularia californica (Hook. & Arn.) Nutt.;Senecio integerrimus Nutt. var exaltatus (Nutt.) Cronq.
DOI:
doi:10.5061/dryad.kc75n
摘要:
. Conclusions: Although here we tested this approach by sequencing plastomes, long PCR amplicons could be generated using DNA from any genome, expanding
Data from: Conflicting phylogenomic signals reveal a pattern of reticulate evolution in a recent high-Andean diversification (Asteraceae: Ast
负责人:
关键词:
reticulate evolution;Astereae;Asteraceae;ddRAD;hybridization;rapid diversification;Diplostephium;introgression;phylogenomics;genome skimming
DOI:
doi:10.5061/dryad.cn74h
摘要:
luding simulations and the detection of introgression using the D-statistic (ABBA-BABA test). We found significant incongruence among the nuclear, chloroplast
Data from: Phylogenetic utility of ycf1 in orchids: a plastid gene more variable than matK
负责人:
关键词:
Orchidaceae;nrITS;matK;phylogeny;molecular systematics;ycf1;Chloroplast
DOI:
doi:10.5061/dryad.q2s7h
摘要:
of the branches). Here, we demonstrate the phylogenetic utility of a heretofore unused portion of a plastid protein-coding gene, hypothetical chloroplast
Data from: Where did all the trees come from? A novel multispecies approach reveals the impacts of biogeographical history and func
负责人:
关键词:
refugia;chloroplast genome;Australian Rain Forest Species;single nucleotide polymorphisms;Next Generation Sequencing;Functional traits
DOI:
doi:10.5061/dryad.j64j0
摘要:
ical rain forests in south-eastern Australia: Nightcap–Border Ranges, Dorrigo and Washpool. Methods: We assembled chloroplast genomic data from whole-genome shotgun libraries for 71 speci

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