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Data from: Single-molecule RNA detection at depth by hybridization chain reaction and tissue hydrogel embedding and clearing
负责人:
关键词:
amplification;CLARITY;single molecule RNA
DOI:
doi:10.5061/dryad.31r0d
摘要:
Accurate and robust detection of mRNA molecules in thick tissue samples can reveal gene expression patterns in single cells within their native
Data from: Local molecular clocks in three nuclear genes: divergence times for rodents and other mammals and incompatibility among fossil calibrations.
负责人:
关键词:
Placentalia;vWF;Rodentia;A2AB;Eutheria;Mammalia;Supermatrix;IRBP;Glires;phylogeny;molecular dating;maximum likelihood;Cenozoic
DOI:
doi:10.5061/dryad.8502
摘要:
of molecules is the evolutionary rate variation among lineages, precluding the application of the global molecular clock. We considered 2422 first and sec
Data from: Direct sequencing of haplotypes from diploid individuals through a modified emulsion PCR-based single-molecule sequencing approach
负责人:
关键词:
Eurtytemora affinis
DOI:
doi:10.5061/dryad.rh7f4
摘要:
starting DNA template molecule, and thus a single haplotype. We improved upon previous approaches by including SYBR Green I and a melted agarose solution
Data from: Delimiting species-poor datasets using single molecular markers: a study of barcode gaps, haplowebs and GMYC
负责人:
关键词:
species delimitation;haplowebs;Heterozygosity;barcode gap;generalized mixed Yule-coalescent model;taxonomy;systematics;molecular markers
DOI:
doi:10.5061/dryad.t7m5v
摘要:
Most single-locus molecular approaches to species delimitation available to date have been designed and tested on data sets comprising at least tens
Data from: Single-molecule imaging and quantification of the immune-variant adhesin VAR2CSA on knobs of Plasmodium falciparum-infected erythrocytes
负责人:
关键词:
sickle hemoglobin;Plasmodium falciparum;knob;PALM;VAR2CSA;single molecule counting
DOI:
doi:10.5061/dryad.39jf355
摘要:
methods. We show that knobs contain 3.3?±?1.7 and 4.3?±?2.5 PfEMP1 molecules, predominantly placed on the knob tip, in parasitized erythrocytes containi
Data from: Crowding induces entropically-driven changes to DNA dynamics that depend on crowder structure and ionic conditions
负责人:
关键词:
single-molecule tracking;macromolecular crowding;entropic forces;DNA conformation;DNA diffusion;depletion interactions;DNA Compaction;fluorescence microscopy
DOI:
doi:10.5061/dryad.77g469g
摘要:
single-molecule fluorescence microscopy and custom particle-tracking algorithms to investigate the impact of varying macromolecular crowding conditions on the transport
Data from: Comparison of methods for molecular species delimitation across a range of speciation scenarios
负责人:
关键词:
molecular species delimitation;Multispecies coalescent;Bayesian phylogenetics;Simulation;generalized mixed Yule-coalescent;Poisson tree processes;speciation
DOI:
doi:10.5061/dryad.739bs
摘要:
molecular approaches for species delimitation. Among the most widely used methods, the generalized mixed Yule-coalescent (GMYC) and Poisson tree processes (PTP) wer
Data from: Stem cell differentiation trajectories in Hydra resolved at single-cell resolution
负责人:
Siebert, Stefan
关键词:
Hydra URD Drop-seq single-cell Seq stem cell differentiation trajectory
DOI:
doi:10.5061/dryad.v5r6077
摘要:
this homeostatic tissue maintenance are not well understood. We sequenced 24,985 Hydra single-cell transcriptomes and identified the molecular si
Data from: An experimental study of the putative mechanism of a synthetic autonomous rotary DNA nanomotor
负责人:
关键词:
strand displacement;rotary motor;molecular machine;DNA nanotechnology
DOI:
doi:10.5061/dryad.sj179
摘要:
-molecule level. The rotary motor concept presented here has potential applications in molecular processing, DNA computing, biosensing and photonics.
Data from: Automated single particle detection and tracking for large microscopy datasets
负责人:
关键词:
fluoresence;PALM;microscopy;particle detection;Tracking
DOI:
doi:10.5061/dryad.6kg29
摘要:
of cellular components from organelles to single molecules. We begin with validating the performance of our method on synthetic image data, and then extend

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