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Data from: Single-molecule RNA detection at depth by hybridization chain reaction and tissue hydrogel embedding and clearing
- 负责人:
- DOI:
- doi:10.5061/dryad.31r0d
- 摘要:
- Accurate and robust detection of mRNA molecules in thick tissue samples can reveal gene expression patterns in single cells within their native
Data from: Local molecular clocks in three nuclear genes: divergence times for rodents and other mammals and incompatibility among fossil calibrations.
- 负责人:
- DOI:
- doi:10.5061/dryad.8502
- 摘要:
- of molecules is the evolutionary rate variation among lineages, precluding the application of the global molecular clock. We considered 2422 first and sec
Data from: Direct sequencing of haplotypes from diploid individuals through a modified emulsion PCR-based single-molecule sequencing approach
- 负责人:
- 关键词:
- Eurtytemora affinis
- DOI:
- doi:10.5061/dryad.rh7f4
- 摘要:
- starting DNA template molecule, and thus a single haplotype. We improved upon previous approaches by including SYBR Green I and a melted agarose solution
Data from: Delimiting species-poor datasets using single molecular markers: a study of barcode gaps, haplowebs and GMYC
- 负责人:
- DOI:
- doi:10.5061/dryad.t7m5v
- 摘要:
- Most single-locus molecular approaches to species delimitation available to date have been designed and tested on data sets comprising at least tens
Data from: Single-molecule imaging and quantification of the immune-variant adhesin VAR2CSA on knobs of Plasmodium falciparum-infected erythrocytes
- 负责人:
- DOI:
- doi:10.5061/dryad.39jf355
- 摘要:
- methods. We show that knobs contain 3.3?±?1.7 and 4.3?±?2.5 PfEMP1 molecules, predominantly placed on the knob tip, in parasitized erythrocytes containi
Data from: Crowding induces entropically-driven changes to DNA dynamics that depend on crowder structure and ionic conditions
- 负责人:
- DOI:
- doi:10.5061/dryad.77g469g
- 摘要:
- single-molecule fluorescence microscopy and custom particle-tracking algorithms to investigate the impact of varying macromolecular crowding conditions on the transport
Data from: Comparison of methods for molecular species delimitation across a range of speciation scenarios
- 负责人:
- DOI:
- doi:10.5061/dryad.739bs
- 摘要:
- molecular approaches for species delimitation. Among the most widely used methods, the generalized mixed Yule-coalescent (GMYC) and Poisson tree processes (PTP) wer
Data from: Stem cell differentiation trajectories in Hydra resolved at single-cell resolution
- 负责人:
- Siebert, Stefan
- DOI:
- doi:10.5061/dryad.v5r6077
- 摘要:
- this homeostatic tissue maintenance are not well understood. We sequenced 24,985 Hydra single-cell transcriptomes and identified the molecular si
Data from: An experimental study of the putative mechanism of a synthetic autonomous rotary DNA nanomotor
- 负责人:
- DOI:
- doi:10.5061/dryad.sj179
- 摘要:
- -molecule level. The rotary motor concept presented here has potential applications in molecular processing, DNA computing, biosensing and photonics.
Data from: Automated single particle detection and tracking for large microscopy datasets
- 负责人:
- DOI:
- doi:10.5061/dryad.6kg29
- 摘要:
- of cellular components from organelles to single molecules. We begin with validating the performance of our method on synthetic image data, and then extend