Data from: Stem cell differentiation trajectories in Hydra resolved at single-cell resolution

负责人：

Siebert, Stefan

关键词：

URD Hydra Drop-seq single-cell Seq stem cell differentiation trajectory

DOI：

doi:10.5061/dryad.v5r6077

摘要：

The adult Hydra polyp continually renews all of its cells using three separate stem cell populations, but the genetic pathways enabling this homeostatic tissue maintenance are not well understood. We sequenced 24,985 Hydra single-cell transcriptomes and identified the molecular signatures of a broad spectrum of cell states, from stem cells to terminally differentiated cells. We constructed differentiation trajectories for each cell lineage and identified gene modules and putative regulators expressed along these trajectories, thus creating a comprehensive molecular map of all developmental lineages in the adult animal. In addition, we built a gene expression map of the Hydra nervous system. Our work constitutes a resource for addressing questions regarding the evolution of metazoan developmental processes and nervous system function.