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Data from: Efficient in situ barcode sequencing using padlock probe-based BaristaSeq
负责人:
关键词:
in situ sequencing
DOI:
doi:10.5061/dryad.5dd92
摘要:
approaches to reading out cellular barcodes trade off spatial resolution with throughput. Bulk sequencing achieves high throughput but sacrifices
Data from: The QRS (Quantification of Representative Sequences) pipeline for amplicon sequencing: case study on within-population ITS1
负责人:
关键词:
cloning\/sanger sequencing;ITS1 region;Caullerya mesnili;Amplicon Sequencing
DOI:
doi:10.5061/dryad.v7f7r
摘要:
Next generation sequencing (NGS) platforms are replacing traditional molecular biology protocols like cloning and Sanger sequencing. Howeve
Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms
负责人:
关键词:
Genomics\/Proteomics;Natural Selection and Contemporary Evolution;phylogeography;molecular evolution;quantitative genetics;Ecological Genetics
DOI:
doi:10.5061/dryad.7tb72
摘要:
associated DNA sequencing (RAD sequencing or RADseq) has become an established method for SNP genotyping on Illumina sequencing platforms. Her
Data from: Efficiency of ddRAD target enriched sequencing across spiny rock lobster species (Palinuridae: Jasus)
负责人:
关键词:
Jasus edwardsii;variant-calling file;probe sequences;Sagmariasus verreauxi;probes sequences
DOI:
doi:10.5061/dryad.3dk40
摘要:
Double digest restriction site-associated DNA sequencing (ddRADseq) and target capture sequencing methods are used to explore population
Data from: Special features of RAD Sequencing data: implications for genotyping
负责人:
关键词:
Genomics\/Proteomics;Caenorhabditis elegans;Population Ecology;Bioinfomatics\/Phyloinfomatics;Ecological Genetics;Heliconius melpomene
DOI:
doi:10.5061/dryad.218p2
摘要:
RAD Sequencing (RAD-Seq) is an economical and efficient method for SNP discovery and genotyping. As with other sequencing-by-synthesis methods
Data from: RADcap: sequence capture of dual-digest RADseq libraries with identifiable duplicates and reduced missing data
负责人:
关键词:
Target Enrichment;Reduced Representation Libraries;3RAD;ddRAD;Wisteria;Illumina
DOI:
doi:10.5061/dryad.ss6c9
摘要:
methods, such as restriction site associated DNA sequencing (RADseq) and sequence capture, are constrained by costs associated with inefficient use
Data from: Unforeseen consequences of excluding missing data from next-generation sequences: simulation study of RAD sequences
负责人:
关键词:
RADtags;species delimitation;phylogenetic;Next-generation sequencing;RADseq;phylogeography
DOI:
doi:10.5061/dryad.jf361
摘要:
simulations, focusing specifically on RAD sequences, to highlight some of the unforeseen consequence of excluding missing data from next-generation sequencing
Data from: Genotype-free estimation of allele frequencies reduces bias and improves demographic inference from RADSeq data
负责人:
关键词:
ANGSD;Ficedula hypoleuca;genotyping error;RADseq;demographic inference;allele frequencies
DOI:
doi:10.5061/dryad.1sd556t
摘要:
Restriction-site associated sequencing (RADSeq) facilitates rapid generation of thousands of genetic markers at relatively low cost; however, severa
Data from: Sequencing historical specimens: successful preparation of small specimens with low amounts of degraded DNA
负责人:
关键词:
Museum specimens;Bembidion;insects;Coleoptera;high-throughput sequencing;Insecta;natural history collections;Carabidae;Sample Preparation;type specimens;Lionepha
DOI:
doi:10.5061/dryad.31g05
摘要:
Despite advances that allow DNA sequencing of old museum specimens, sequencing small-bodied, historical specimens can be challenging and unreliable
Data from: Reconstructing phylogeny from reduced-representation genome sequencing data without assembly or alignment
负责人:
关键词:
Quercus;alignment-free;assembly-free;RADseq;phylogenomics;missing data
DOI:
doi:10.5061/dryad.r0hq0
摘要:
Reduced-representation genome sequencing such as RADseq aids the analysis of genomes by reducing the quantity of data, thereby lowering bot

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