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Data from: Substantial differences in bias between single-digest and double-digest RAD-seq libraries: a case study
负责人:
关键词:
selection components analysis;Syngnathus scovelli;Next-generation sequencing;allele dropout;PCR duplicates
DOI:
doi:10.5061/dryad.qf916
摘要:
ng sdRAD?seq versus ddRAD?seq. Our finding that sdRAD?seq and ddRAD?seq can result in different allele frequencies has implications for comparisons ac
Data from: Genotyping-in-Thousands by sequencing (GT-seq) panel development and application to minimally-invasive DNA samples to support studies
负责人:
关键词:
conservation genetics;Reptiles;Non-invasive sampling;Crotalus oreganus;targeted amplicon sequencing;SNP panel;molecular ecology;RAD-seq;Wildlife Management
DOI:
doi:10.5061/dryad.vr300p0
摘要:
ciated DNA sequencing (RADseq) assembly. Comparisons of genotypes generated within and among RADseq and GT-seq for the same individuals found low rates
Data from: Trans-Pacific RAD-Seq population genomics confirms introgressive hybridization in Eastern Pacific Pocillopora corals.
负责人:
关键词:
restriction-site associated DNA sequencing (RAD-seq);Transcriptomics;Reef corals;phylogeography;population genomics;hybridization;Holocene;Pocillopora damicornis;P. elegans;P. eydouxi
DOI:
doi:10.5061/dryad.436h0
摘要:
introgressive hybridization. Here we used 6769 genome-wide SNPs from Restriction-site Associated DNA sequencing (RAD-Seq) to conduct phylogenomic comparisons among three common
Data from: Genomic scans reveal multiple mito?nuclear incompatibilities in population crosses of the copepod Tigriopus californicus
负责人:
关键词:
postzygotic reproductive isolation;Hybrid inviability;Tigriopus;Pool-Seq;Tigriopus californicus
DOI:
doi:10.5061/dryad.0jp4740
摘要:
-nuclear) versus those between mitochondrial and nuclear factors (mito-nuclear) in a species that lacks sex chromosomes. We used a Pool-seq approach
Data from: Bioinformatic processing of RAD-seq data dramatically impacts downstream population genetic inference
负责人:
关键词:
genomics;reference genome;ddRAD;bioinformatics;Galápagos sea lion (Zalophus wollebaeki)
DOI:
doi:10.5061/dryad.q14c1
摘要:
Restriction site-associated DNA sequencing (RAD-seq) provides high-resolution population genomic data at low cost, and has become an importa
Data from: The neuropeptide Drosulfakinin regulates social isolation-induced aggression in Drosophila
负责人:
关键词:
aggression;Social Isolation;Cholecystokinin;Drosulfakinin;drosophila melanogaster;neuropeptide
DOI:
doi:10.5061/dryad.m1674h1
摘要:
lation-driven changes in animal behavior and gene expression in the brain. RNA-seq identified several head-expressed genes strongly responding to social iso
Data from: A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife
负责人:
关键词:
NGS RNA-Seq 16S metagenomics metabarcoding 454-pyrosequencing MiSeq bacteria rodent-borne zoonoses coinfections pathobiome
DOI:
doi:10.5061/dryad.50125
摘要:
s not been assessed. Here, we compared two NGS approaches, RNA-Sequencing (RNA-Seq) and 16S-metagenomics, assessing their ability to survey neglected zoonotic bacteria
Data from: Rapid divergence of mussel populations despite incomplete barriers to dispersal
负责人:
关键词:
Isolation-by-distance;population genomics;ddRAD-seq;marine biodiversity;Isolation-by-Environment;Coral Triangle;Brachidontes sp.
DOI:
doi:10.5061/dryad.1r89hq8
摘要:
e found. We hypothesize that (incomplete) dispersal barriers can cause initial isolation, allowing priority effects to give the numerical advantage necessary to initiate
Data from: Population genomic evidence for multiple Pliocene refugia in a montane-restricted harvestman (Arachnida, Opiliones, Sclerobunus robustus) from the southwestern United States
负责人:
关键词:
landscape genomics;Sclerobunus robustus;isolation by environment;sky islands;refugial persistence;ddRAD-seq;Pliocene;Pleistocene
DOI:
doi:10.5061/dryad.bq68f
摘要:
ng ecological niche models (ENMs) across three time periods, corroborated these refugia with population genetic methods using double-digest RAD-seq data and conducted
Data from: A whole-transcriptome approach to evaluating reference genes for quantitative gene expression studies: a case study in Mimulus
负责人:
关键词:
reference genes;Mimulus luteus var. luteus;RNA-seq;normalization;quantitative RT-PCR;expression stability;Mimulus luteus;Mimulus guttatus
DOI:
doi:10.5061/dryad.84655
摘要:
, including emerging and nonmodel systems. We find that RNA-seq accurately estimates gene expression means in comparison to qPCR, and that expression means ar

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