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Data from: A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife
- 负责人:
- 关键词:
- NGS RNA-Seq 16S metagenomics metabarcoding 454-pyrosequencing MiSeq bacteria rodent-borne zoonoses coinfections pathobiome
- DOI:
- doi:10.5061/dryad.50125
- 摘要:
- s not been assessed. Here, we compared two NGS approaches, RNA-Sequencing (RNA-Seq) and 16S-metagenomics, assessing their ability to survey neglected zoonotic bacteria
Data from: Bacterial characterization of Beijing drinking water by flow cytometry and MiSeq sequencing of the 16S rRNA gene
- 负责人:
- DOI:
- doi:10.5061/dryad.55nb0
- 摘要:
- Flow cytometry (FCM) and 16S rRNA gene sequencing data are commonly used to monitor and characterize microbial differenc
Data from: Rapid species-level identification of vaginal and oral lactobacilli using MALDI-TOF MS analysis and 16S rDNA sequencing
- 负责人:
- DOI:
- doi:10.5061/dryad.78vs2
- 摘要:
- to identify lactobacilli to the species level. Even though some results were ambiguous while 16S rDNA sequencing yielded confident speci
Data from: Phylogenetic relatedness determined between antibiotic resistance and 16S rRNA genes in actinobacteria
- 负责人:
- DOI:
- doi:10.5061/dryad.td742
- 摘要:
- , distances of erm gene sequences were significantly correlated to phylogenetic distances based on 16S rRNA genes, while ABC transporter gene distances wer
Data from: COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)
- 负责人:
- Xia, Yun
- DOI:
- doi:10.5061/dryad.p4r84
- 摘要:
- to be highly variable in amphibians, sequencing is often challenging. Consequently, another mtDNA gene, 16S rRNA gene, is often advocated for amphibian barcoding
Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility
- 负责人:
- Porter, Teresita
- DOI:
- doi:10.5061/dryad.4m7d1
- 摘要:
- DNA sequencing of ancient permafrost samples can be used to reconstruct past plant, animal and bacterial communities. In this study, we asse
Data from: ITS all right mama: Investigating the formation of chimeric sequences in the ITS2 region by DNA metabarcoding analyses of fungal mock
- 负责人:
- DOI:
- doi:10.5061/dryad.n4cv3
- 摘要:
- and 454 sequencing of the ITS2 region from fungal mock communities of different complexities. We evaluated the chimera detecting ability of two common chimera-checking
Data from: Drivers and patterns of microbial community assembly in a Lyme disease vector
- 负责人:
- DOI:
- doi:10.5061/dryad.2nv32qh
- 摘要:
- understanding of the processes shaping the vector microbiome and the interactions therein. Here we use 16S rRNA sequencing and apply a community ecology framework
Data from: A genetic discontinuity in root-nodulating bacteria of cultivated pea in the Indian trans-Himalayas
- 负责人:
- DOI:
- doi:10.5061/dryad.21rt0fp0
- 摘要:
- of the trans-Himalayan valleys of Lahaul and Spiti in the state of Himachal Pradesh of India were studied using 16S rRNA gene PCR-RFLP, ERIC-PCR, sequencing
Data from: Kinship, inbreeding, and fine-scale spatial structure influence gut microbiota in a hindgut-fermenting tortoise
- 负责人:
- DOI:
- doi:10.5061/dryad.54bm8
- 摘要:
- in an obligate herbivorous reptile. Using multiplexed 16S rRNA gene sequencing, we characterized the faecal microbial community of a population of gopher tortoise