Data from: A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife

负责人：

关键词：

NGS RNA-Seq 16S metagenomics metabarcoding 454-pyrosequencing MiSeq bacteria rodent-borne zoonoses coinfections pathobiome

DOI：

doi:10.5061/dryad.50125

摘要：

. DNA extracts were analyzed via a 16S-metagenomics approach using two sequencers: the 454 GS-FLX and the MiSeq. The V4 region of the gene coding for 16S rRNA

Data from: Reproductive and behavior dysfunction induced by maternal androgen exposure and obesity is likely not gut microbiome-mediated

负责人：

关键词：

maternal androgen excess;2016-2018;maternal obesity;Mice;gutmicrobiota;Mice;Polycystic ovary syndrome

DOI：

doi:10.5061/dryad.7h4338m

摘要：

in the gut microbiome of offspring. Fecal microbiome profiles were assessed using Illumina paired-end sequencing of 16S rRNA gene V4 amplicons. We found sex-specific

Data from: Microbiome changes through ontogeny of a tick pathogen vector

负责人：

关键词：

blacklegged tick;16S rRNA;Borrelia;Wolbachia;developmental stages;Bacterial Community;Anaplasma;Rickettsia;microbiome;Holocene;Ixodes scapularis

DOI：

doi:10.5061/dryad.mt6m0

摘要：

high-throughput sequencing of the 16S rRNA gene V3-V4 hypervariable regions in the first study to investigate the tick microbiome across all developmental stages (larvae

Data from: Comparing microbiota profiles in induced and spontaneous sputum samples in COPD patients

负责人：

关键词：

Homo Sapiens;sputum;marker-gene study;COPD

DOI：

doi:10.5061/dryad.5gc82

摘要：

sample pairs. DNA was extracted by enzymatic and mechanical lysis methods. The V3-V4 region of the 16S rRNA gene was PCR-amplified and prepared for paired

Data from: Seasonal and ontogenetic variation of skin microbial communities and relationships to natural disease dynamics in declining amphibians

负责人：

关键词：

16S rRNA V4 sequences;Lithobates yavapaiensis;chytridiomycosis;Eleutherodactylus coqui;microbial communities;Batrachochytrium dendrobatidis;Amphibians

DOI：

doi:10.5061/dryad.7v81b

摘要：

d a combination of community-fingerprinting analyses and 16S rRNA amplicon sequencing to quantify changes in bacterial diversity and assemblage composition bet

Data from: Temperature variation, bacterial diversity, and fungal infection dynamics in the amphibian skin

负责人：

关键词：

Environmental DNA;Species Interactions;16S V4 rRNA amplicon sequencing;2012;Eleutherodactylus coqui;bacteria;Holocene;Batrachochytrium dendrobatidis;Amphibians;Disease Biology;population dynamics

DOI：

doi:10.5061/dryad.hc321

摘要：

Host-associated bacterial communities on the skin act as the first line of defence against invading pathogens. Yet, for most natural systems, we lack