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Pectate liases, nucleic acids that encode them and methods for their preparation and use
专利权人:
BASF ENZYMES LLC
发明人:
KEROVUO, JANNE,SOLBAK, ARNE,GRAY, KEVIN,MCCANN, RYAN,PUROHIT, SHALAKA,GERENDASH, JOEL,JANSSEN, GISELLE,DAHOD, SAMUN
申请号:
ES04758802
公开号:
ES2545639T3
申请日:
2004.04.02
申请国别(地区):
ES
年份:
2015
代理人:
摘要:
An isolated, synthetic, or recombinant nucleic acid comprising (a) a nucleic acid sequence having a sequence identity of at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or greater or 100% identical to SEQ ID NO: 77, over a region of at least 950, 1000, 1050, 1100, 1150 or more remains, or the full length of a gene or a transcript, in which the nucleic acid encodes at least one polypeptide having a pectate lyase activity; (b) a nucleic acid sequence comprising a sequence as set forth in SEQ ID NO: 77; (c) a nucleic acid sequence encoding a polypeptide having a sequence as set forth in SEQ ID NO: 78, SEQ ID NO: 132 or SEQ ID NO: 134; (d) a sequence that hybridizes under stringent conditions with a nucleic acid comprising SEQ ID NO: 77, SEQ ID NO: 131 or SEQ ID NO: 133, wherein the nucleic acid encodes a polypeptide having an activity of pectate lyase, in which the stringent conditions include a washing step comprising a washing in 0.2X SSC at a temperature of about 65 ° C for about 15 minutes; (e) the nucleic acid of (a), (b), (c) or (d) that encodes a lycta pectate but lacks a signal sequence, a prepro domain, or a combination thereof; (f) the nucleic acid of (a), (b), (c), (d) or (e) that encodes a lycta pectate but also has a heterologous sequence; or (g) a complementary sequence with (a), (b), (c), (d), (e), or (f).Un ácido nucleico aislado, sintético, o recombinante que comprende (a) una secuencia de ácidos nucleicos que tiene una identidad de secuencia de al menos un 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 %, 99 %, o superior o que es idéntica en un 100 % a la SEC ID Nº: 77, sobre una región de al menos 950, 1000, 1050, 1100, 1150 o más restos, o la longitud completa de un gen o de un transcrito, en la que el ácido nucleico codifica al menos un polipéptido que tiene una actividad de pectato liasa; (b) una secuencia de ácidos nucleicos que comprende una secuencia como se establece en la SEC ID Nº: 77; (c) una secuencia de ácidos nucleicos qu
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