STEER, BRIAN,DYCAICO, MARK,KLINE, KATIE,TREFZER, AXEL,TODARO, TOM,SOLBAK, ARNE,EL-FARRAH, FATIMA,ALVARADO, ALBERTO,FREY, GERHARD
申请号：
ES11181439
公开号：
ES2531135T3
申请日:
2007.09.21
申请国别(地区):
ES
年份:
2015
代理人:
摘要：
An isolated, synthetic or recombinant nucleic acid comprising (a) a nucleic acid sequence encoding a polypeptide having a phytase activity and having at least 95%, 96%, 97%, 98% or 99% or more identity of sequence with SEQ ID NO: 1, and having a nucleotide modification where the nucleotides at positions 1045 to 1047 of SEQ ID NO: 1 are changed to TAT OR TAC, where the polypeptide has improved thermostability over the phytase of SEQ ID NO. : 2； (b) a nucleic acid encoding a polypeptide that has a phytase activity and that has at least 95%, 96%, 97%, 98% or 99% or more sequence identity of SEQ ID NO: 2, and that has a amino acid modification where the amino acid threonine at amino acid position 349 of SEQ ID NO: 2 is replaced by a tyrosine, where the polypeptide has improved thermostability over the phytase of SEQ ID NO: 2； (c) the nucleic acid sequence of (a) or (b) encoding a polypeptide that has a phytase activity but lacks: a signal sequence or proprotein sequence, or a homologous promoter sequence； (d) the nucleic acid of any of (a) to (c) that encodes a polypeptide that has a phytase activity and that further comprises a heterologous amino acid sequence, or the nucleic acid of any of (a) to (c) that further comprises a heterologous nucleotide sequence； (e) the nucleic acid of (d), where the heterologous amino acid sequence comprises, or consists of a sequence that encodes a heterologous (leader) signal sequence, or a marker or an epitope, or the heterologous nucleotide sequence comprising a heterologous promoter or encodes a target sequence； or (f) a nucleic acid sequence completely complementary to the nucleic acid sequence of any of (a) to (e).Un ácido nucleico aislado, sintético o recombinante que comprende (a) una secuencia de ácido nucleico que codifica un polipéptido que tiene una actividad fitasa y que tiene al menos 95%, 96%, 97%, 98% o 99% o más identidad de secuencia con SEC ID Nº: 1, y que tiene una modificación de nucleótidos donde los nucleótidos en
