A transplanting device separates a seedling bed piece with a seedling from a seedling bed along slits and transplants the separated seedling bed piece to a permanent planting panel. The transplanting device includes a separating mechanism and a transplanting mechanism. The separating mechanism includes a holding member and is capable of separating the seedling bed piece from the seedling bed. The transplanting mechanism is capable of transplanting the separated seedling bed piece to the permanent planting panel. The separating mechanism includes an engagement bar capable of hampering movement of an adjacent seedling bed piece adjacent to a holding-target seedling bed piece held by the holding member by becoming engaged with the adjacent seedling bed piece from a side that is different from a first side, on which the stalk of the seedling grows.