[Problem] To reduce the possibility of damaging seedlings in association with transplantation of unit seedling beds in which seedlings having geminated from seeds are separately planted, in transplantation from a seedling tray supporting the unit seedling beds, to a hydroponic panel where a larger distance can be ensured between the seedlings. [Solution] A transplantation device 1 includes: a seedling tray 3 that is supported by a frame 2 and that supports a seedling bed 7 in which mutually separatable unit seedling beds 71 are arranged in two directions on a plane; a hydroponic panel 4 that is supported by the frame 2, that is a transplantation destination of the unit seedling beds 71 on the seedling tray 3, and that has formed therein holes 41 into which the unit seedling beds 71 are housed; and a holding member 5 that is supported by the frame 2 so as to be able to freely rotate about the vertical axis and be freely elevated/lowered, that holds any one or more unit seedling beds 71 arranged at the most outer peripheral side of the seedling bed 7 on the seedling tray 3, from a side surface side of the unit seedling beds 71 toward the width direction of the unit seedling beds 71, and separates the unit seedling beds 71 from the seedling bed 7, and inserts the held unit seedling beds 71 into the holes 41 of the hydroponic panel 4.