SYNTHETIC DNA ENCODING THE ANTI-MULLERIAN HUMAN HORMONE, EXPRESSION VECTOR pTVK4pu/MISOPT THAT CONTAINS THIS DNA, CHINESE HAMSTER OVARY CHO-MIS CELLS STRAIN - RECOMBINANT ANTI-MULLERIAN HUMAN HORMONE PRODUCER
Petrov Aleksandr Vladimirovich,Karasev Maksim Mikhajlovich,Kudling Tatyana Viktorovna,Pigareva Natalya Vasilevna,Sergeeva Valeriya Evgenevna,Trofimov Aleksandr Viktorovich,Ishchenko Aleksandr Mitrofanovich,Gorbunov Aleksej Andreevich,Vakhrushev Andrej Valentinovich,Simbirtsev Andrej Semenovich
申请号：
RU20160115498
公开号：
RU2616273(C1)
申请日:
2016.04.21
申请国别(地区):
俄罗斯
年份:
2017
代理人:
摘要：
FIELD: biotechnology.SUBSTANCE: synthetic DNA is prepared that encodes anti-mullerian human hormone, contains anti-mullerian human hormone gene with a codon composition and guanine and cytosine content, optimized for expression in Chinese hamster ovary cells. Based on the obtained DNA expression vector pTVK4pu/MISOPT is constructed, with scheme shown in Fig. 1, which contains a puromycin resistant gene as a selectable marker. pTVK4pu/MISOPT expression vector transforms CHO cells to produce a strain of Chinese hamster ovary cells CHO-MIS - recombinant anti-mullerian human hormone deposited under number RKKK(P) 775D. Strain CHO-MIS provides accumulation of up to 200 mg/l of AMG in the culture medium for 11 days in serum-free chemically defined medium with a composition containing no animal-derived components.EFFECT: possibility of application for drug development.3 cl, 7 dwg, 2 tbl, 4 ex
