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OBTAINING A TRANSPLANT FROM ANIMAL DERMIS USING SODIUM SULPHIDE SOLUTION
专利权人:
ТУТОГЕН МЕДИКАЛ ГМБХ (DE)
发明人:
ВИЛЬХЕЛЬМИ Арнд (DE),ШРАЙНЕР Зильке (DE)
申请号:
RU2013137859/15
公开号:
RU2013137859A
申请日:
2012.01.12
申请国别(地区):
RU
年份:
2015
代理人:
摘要:
1. A method of obtaining a transplant from an animal’s dermis, preferably from a pig’s dermis, comprising the following steps: a) preparing an animal’s dermis; b) treating the animal’s dermis with an aqueous solution containing sodium sulfide; c) one or multiple treatment of the dermis with aqueous saline; d) if necessary, single or multiple treatment of the dermis with an aqueous solution of hydrogen peroxide; e) dehydration of the dermis. 2. The method according to claim 1, characterized in that in step b) an aqueous alkaline solution containing sodium sulfide is used. The method according to claim 1 or 2, characterized in that in step b) an aqueous solution is used containing from 0.01 to 10% by weight, preferably from 0.1 to 5% by weight, more preferably from 0.5 to 3 % wt., most preferably from 0.75 to 1.5% of the mass. and highly preferably 1% of the mass. sodium sulfide. 4. The method according to claim 1, characterized in that in step b) an aqueous solution is used which, along with sodium sulfide, contains from 0.001 to 0.5 M, preferably from 0.005 to 0.4 M, particularly preferably from 0.01 to 0, 3 M, more preferably 0.05 to 0.2 M, most preferably 0.075 to 0.125 M, and most preferably 1 M sodium hydroxide. 5. The method according to claim 1, characterized in that the dermis in stage b) for 1 to 48 hours, preferably 5 to 36 hours, more preferably 12 to 24 hours and most preferably 14 to 20 hours, is treated with an aqueous solution containing sodium sulfide, in an amount of from 5 to 15 times the volume, preferably from 6 to 14 times the volume, particularly preferably from 7 to 13 times the volume, more preferably from 8 to 12 times the volume and most preferably from 9 - up to 11-fold volume per dermis. 6. Method p1. Способ получения трансплантата из дермы животного, предпочтительно из дермы свиньи, включающий в себя следующие стадии:a) подготовка дермы животного;b) обработка дермы животного водным раствором, содержащим сульфид натрия;c) одно- или многокр
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