There are provided an insect-resistant protein exhibiting a sufficient resistance to insects even in a small amount; an insect-resistance gene encoding said insect-resistant protein; a recombinant vector containing said insect-resistance gene; a host cell and a plant cell having the recombinant vector transferred thereinto; a transformant transformed by the insect-resistance gene and a method for producing the same; a protein recovered thereby; and an insect-resistant agent comprising them as active ingredients. In accordance with the present invention, the insect-resistant protein derived from a plant, comprises a first amino acid sequence having a 50% or more homology with the amino acid sequence represented by SEQ ID NO:1 in Sequence Listing, a second amino acid sequence having a 50% or more homology with the amino acid sequence represented by SEQ ID NO:2 in Sequence Listing and a third amino acid sequence having a 50% or more homology with the amino acid sequence represented by SEQ ID NO: 3 in Sequence Listing, wherein the second amino acid sequence has at least one spppp sequence.