CYCLACEL LIMITED;CRAIG, ROGER, KINGDON;GREEN, SIMON;COLYER, JOHN
发明人:
CRAIG, ROGER, KINGDON,GREEN, SIMON,COLYER, JOHN
申请号：
WO2000GB04348
公开号：
WO0136617(A3)
申请日:
2000.11.15
申请国别(地区):
世界知识产权组织国际局
年份:
2002
代理人:
摘要：
A method is provided for measuring <i>in vivo</i> in a transgenic non-human multicellular organism the activity of a cellular enzyme, which organism is transgenic by virtue of comprising one or more nucleic acid constructs encoding a binding domain and a binding partner thereof, wherein: (i) the binding domain and/or binding partner comprise a site subject to post-translational modification by the cellular enzyme; (ii) modification of the site by the enzyme affects the interaction between the binding domain and binding partner; and (iii) the binding domain and binding partner each comprise a detectable label such that when the binding domain and binding partner interact, a detectable physical characteristic of one or both of the labels is altered, which method comprises measuring the interaction between the binding domain and the binding partner by measuring changes in said physical characteristic in one or more cells of the transgenic organism. A transgenic non-human multicellular organism is also provided.
